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目的:建立叶下珠药材的高效液相色谱指纹图谱。方法:采用反相高效液相色谱法,以柯里拉京为内参比峰。色谱条件为Kromasil C18柱(4.6 mm×250 mm,5μm),流动相乙腈-体积分数为0.1%的磷酸水,梯度洗脱,流速1.0 mL.min-1,柱温30℃,检测波长280 nm。采用SPSS 16.0软件进行系统聚类分析;采用国家药典委员会颁布的中药色谱指纹图谱相似度评价系统(2009版)进行相似度评价。结果:在色谱指纹图谱中,确立了21个共有峰,指认了其中6个色谱峰,系统聚类分析将14批叶下珠药材分为两类,建立了叶下珠药材的共有模式,在14批叶下珠药材中有12批样品的指纹图谱相似度在0.90以上。结论:该方法简便、准确、重复性好,为评价叶下珠药材的质量提供了依据。
Objective: To establish the HPLC fingerprint of Phyllanthus urinaria. Methods: Reversed phase high performance liquid chromatography (HPLC) was used as the internal reference peak. The chromatographic conditions were Kromasil C18 column (4.6 mm × 250 mm, 5 μm) and mobile phase of acetonitrile-0.1% phosphoric acid with a gradient elution at a flow rate of 1.0 mL · min-1. The column temperature was 30 ℃ and the detection wavelength was 280 nm . SPSS 16.0 was used to carry out systematic cluster analysis. Similarity evaluation was carried out by using the similarity evaluation system of Chinese traditional medicine chromatography fingerprint (2009 version) promulgated by the State Pharmacopoeia Commission. Results: In chromatographic fingerprinting, 21 common peaks were identified and 6 of them were identified. The phylogenetic tree was divided into two groups according to phylogenetic analysis, and the common pattern of Phyllanthus was established. 14 batches of Phyllanthus medicine in 12 batches of samples fingerprint similarity of 0.90 or more. Conclusion: The method is simple, accurate and reproducible. It provides the basis for evaluating the quality of Phyllanthus urinaria.