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通过克隆外周血淋巴细胞并用６巯基鸟嘌呤筛选次黄嘌呤磷酸核糖转移酶基因（ｈｙｐｏｘａｎｔｈｉｎｅｐｈｏｓｐｈｏｒｉｂｏｓｙｌｔｒａｎｓｆｅｒａｓｅ，ＨＰＲＴ）缺陷的突变细胞，测定了５例急性放射病人在受２．０～５．２Ｇｙ６０Ｃｏγ射线意外照射后５．５年外周血淋巴细胞ＨＰＲＴ基因突变频率。观察到细胞克隆效率、突变细胞克隆数、ＨＰＲＴ突变频率与受照剂量有一定的依赖性。用多引物聚合酶链反应（ＰＣＲ）扩增突变细胞ＨＰＲＴ基因全部９个外显子伴以琼脂糖凝胶电泳以分析基因突变谱。结果表明，高剂量受照病人的外显子缺失总数较低剂量者高。与照后３．５年和４．５年的突变谱比较，外显子缺失突变比例逐年下降，而点突变比例逐年升高。可能反映病人机体的恢复和受损ＤＮＡ的修复。
By means of cloning of peripheral blood lymphocytes and screening of mutant cells deficient in hypoxanthinephosphoribosyltransferase (HPRT) with 6-mercapto-guanine, five patients with acute radiation were tested for their ability to undergo irradiation with 2.0-5.2Gy of 60Coγ-ray Frequency of HPRT mutations in peripheral blood lymphocytes 5.5 years after accidental irradiation. Observed cell cloning efficiency, the number of mutant cell clones, HPRT mutation frequency and the dose has a certain dependence. All nine exons of HPRT gene were amplified by multi-primer polymerase chain reaction (PCR) and analyzed by agarose gel electrophoresis to analyze gene mutation profiles. The results showed that patients with high doses of exon deficiency total number of patients at higher doses. Compared with the mutation spectrum of 3.5 years and 4.5 years after the irradiation, the proportion of exon deletion mutation decreased year by year, while the proportion of point mutation increased year by year. May reflect the recovery of the patient’s body and damaged DNA repair.