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目的考察影响化合物3,5-O-二咖啡酰基奎宁酸稳定性的因素并对3,5-O-二咖啡酰基奎宁酸的体外抗氧化活性进行初步研究。方法采用HPLC考察3,5-O-二咖啡酰基奎宁酸在不同溶剂、pH、温度、光照条件下的稳定性;采用UV测定3,5-O-二咖啡酰基奎宁酸的体外抗氧化活性(清除DPPH自由基)。结果溶剂的种类、pH值、温度、光照条件对3,5-O-二咖啡酰基奎宁酸稳定性具有一定影响。3,5-O-二咖啡酰基奎宁酸具有较强的体外清除DPPH自由基活性,其活性与Vc相当[3,5-O-二咖啡酰基奎宁酸和Vc的IC_(50)值分别为(372.56±1.04)μg·mL~(-1)和(294.54±1.03)μg·mL~(-1)]。结论在该化合物的分离纯化及其分析检测时,不能采用纯有机溶剂为溶剂,应在中性或酸性条件下,低温、避光操作。3,5-O-二咖啡酰基奎宁酸作为抗氧化剂在制药、食品、化妆品以及精细化工行业具有广阔的应用前景。
Objective To investigate the factors that affect the stability of 3,5-O-dis Caffeoylquinic acid and to study the in vitro antioxidant activity of 3,5-O-dis Caffeoylquinic acid. Methods The stability of 3,5-O-dis Caffeoylquinic acid under different solvents, pH, temperature and light conditions was investigated by HPLC. The in vitro antioxidant activity of 3,5-O-dis Caffeoylquinic acid Activity (DPPH free radical scavenging). Results The type of solvent, pH value, temperature and light conditions had certain influence on the stability of 3,5-O-disaccharidinylquinic acid. 3,5-O-dis Caffeoylquinic acid has strong DPPH radical scavenging activity in vitro and its activity is comparable to that of Vc [IC 50 value of 3,5-O-dis Caffeoylquinic acid and V c (372.56 ± 1.04) μg · mL -1 and (294.54 ± 1.03) μg · mL -1), respectively. Conclusion In the separation and purification of the compound and its analytical detection, pure organic solvents can not be used as a solvent, should be in neutral or acidic conditions, low temperature, dark operation. 3,5-O-Dicaffeoylquinic acid as a antioxidant in the pharmaceutical, food, cosmetics and fine chemical industry has broad application prospects.