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以吖啶橙(AO)为荧光探针,应用各种光谱法和热力学方法研究士的宁和布鲁辛与小牛胸腺DNA(ct-DNA)的相互作用情况。实验结果表明,在模拟人体生理环境下,把士的宁和布鲁辛这两种药物加入到DNA-AO体系中时,DNA-AO的光谱特征发生了明显的变化:在200nm和260nm处的紫外光谱吸收峰均有明显的减色效应,但没有红移和蓝移现象,表明药物与DNA不是典型的嵌插作用结合;DNA-AO的荧光光谱被药物显著的猝灭,根据Stern-Volmer方程可计算出士的宁和布鲁辛与DNA的成键常数分别是9.85×103L.mol-1和4.76×103L.mol-1。热变性温度,离子强度法和黏度法进一步证明药物与DNA不是嵌插结合,而是以沟槽作用的方式结合。
Using acridine orange (AO) as a fluorescent probe, the interaction between strychnine and brucein and calf thymus DNA (ct-DNA) was investigated using various spectroscopic and thermodynamic methods. The experimental results showed that the spectral characteristics of DNA-AO changed significantly when strychnine and Brussin were added into the DNA-AO system under simulated human physiological environment: UV at 200nm and 260nm However, there was no redshift and blue shift phenomenon, indicating that drug and DNA were not typical intercalation. Fluorescence spectra of DNA-AO were significantly quenched by drugs. According to the Stern-Volmer equation The bond constants of strychnine and brucellin with DNA were calculated to be 9.85 × 103 L · mol -1 and 4.76 × 103 L · mol -1, respectively. Thermal denaturation temperature, ionic strength and viscosity method to further prove that the drug is not intercalated with DNA binding, but in a way of groove effect.