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目的本研究通过检测骨髓增生异常综合征-难治性贫血(MDS-RA)及难治性贫血伴原始细胞增多(MDS-RAEB)患者骨髓间充质干细胞(MSC)转化生长因子(TGF-β1)、肝细胞生长因子(HGF)的表达,探讨MSC的免疫抑制作用在MDS发病中的意义。方法分离、培养23例MDS患者(MDS-RA组14例,MDS-RAEB组9例)及对照组12例的骨髓间充质干细胞,比较3组MSC的形态,采用实时定量PCR的方法检测骨髓MSC内转化生长因子(TGF-β1)、肝细胞生长因子(HGF)mRNA的表达水平;采用酶联免疫吸附测定法(ELISA)检测骨髓MSC分泌的TGF-β1、HGF的浓度。结果 3组MSC的形态无明显差异,MDS-RA组和MDS-RAEB组MSC表达和分泌TGF-β1、HGF水平明显低于对照组(P均<0.05),MDS-RA组和MDS-RAEB组MSC表达和分泌TGF-β1、HGF无明显差异(P均>0.05)。结论 MDS-RA与MDS-RAEB表达TGF-β1、HGF mRNA的水平及分泌TGF-β1、HGF的浓度水平均较正常减低,导致MSC免疫抑制能力减低,可能与MDS免疫监视低下,出现异常单克隆造血相关。
OBJECTIVE: To detect the expression of TGF-β1 in bone marrow mesenchymal stem cells (MSC) of patients with myelodysplastic syndrome-refractory anemia (MDS-RA) and refractory anemia with proliferative myelocytosis (MDS-RAEB) ), Hepatocyte growth factor (HGF), and explore the significance of MSC immunosuppression in the pathogenesis of MDS. Methods Bone marrow mesenchymal stem cells from 23 MDS patients (MDS-RA group, MDS-RAEB group, 9 cases) and control group (12 cases) were isolated and cultured. The morphology of MSC was compared between the three groups. Real- (TGF-β1) and hepatocyte growth factor (HGF) mRNA levels in MSC were detected by enzyme-linked immunosorbent assay (ELISA). The levels of TGF-β1 and HGF secreted by bone marrow MSCs were detected by enzyme linked immunosorbent assay (ELISA) Results There was no significant difference in the morphology of MSC between the three groups. The levels of TGF-β1 and TGF-β1 secreted by MSCs in MDS-RA group and MDS-RAEB group were significantly lower than those in control group (all P <0.05). MDS-RA group and MDS-RAEB group There was no significant difference in MSC expression and secretion of TGF-β1 and HGF (all P> 0.05). Conclusions The levels of TGF-β1 and HGF mRNA and the levels of TGF-β1 and HGF secreted by MDS-RA and MDS-RAEB are both lower than normal, leading to the decrease of immunosuppressive capacity of MSCs and their low immunosurveillance with MDS. Related to hematopoiesis.