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目的:探讨肺癌患者外周血循环内皮细胞(CECs)包括活性循环内皮细胞(aCECs)与VEGF之间的关系及其在肺癌中的临床意义。方法:用流式细胞仪对70例肺癌患者(NSCLC64例)化疗前后及30例健康人外周血CECs及aCECs进行检测,同时采用ELISA方法检测肺癌患者化疗前后VEGF水平。结果:NSCLC患者外周血CECs及aCECs的数量明显高于健康人群(P=0.000)。不同病理类型肺癌之间CECs及aCECs的数量差异无统计学意义(NSCLC与SCLC,P=0.878、0.936;鳞癌与腺癌,P=0.786、0.658)。CECs及aCECs的数量与VEGF之间无明显相关性(P=0.697、0.631)。治疗后不同疗效间肺癌患者VEGF水平比较,PD均高于CR+PR及SD(P=0.009、0.031)。NSCLC及SCLC化疗后aCECs的数量高于化疗前(P=0.031、0.029);SD及PD患者CECs、aCECs的数量高于化疗前(P=0.024、0.014;P=0.036、0.028);对治疗后不同疗效肺癌患者CECs及aCECs水平比较,aCECs数量PD、SD均高于CR+PR(P=0.022、0.009)。结论:VEGF联合CECs及aCECs检测可以作为很好的预测性指标评估肺癌的血管生成状况及判断预后。
Objective: To investigate the relationship between circulating endothelial cells (CECs) including circulating circulating endothelial cells (aECs) and VEGF in patients with lung cancer and their clinical significance in lung cancer. Methods: Peripheral blood CECs and aCECs were detected by flow cytometry in 70 patients with lung cancer (NSCLC, 64 cases) before and after chemotherapy and 30 healthy people. The levels of VEGF in lung cancer patients before and after chemotherapy were detected by ELISA. Results: The number of CECs and aCECs in peripheral blood of patients with NSCLC was significantly higher than that of healthy people (P = 0.000). There was no significant difference in the number of CECs and aCECs between different pathological types of lung cancer (NSCLC and SCLC, P = 0.878, 0.936; squamous cell carcinoma and adenocarcinoma, P = 0.786,0.658). There was no significant correlation between the number of CECs and aCECs and VEGF (P = 0.697, 0.631). The levels of VEGF in lung cancer patients after treatment were significantly higher than those in CR + PR and SD (P = 0.009,0.031). The number of aCECs in NSCLC and SCLC was higher than those before chemotherapy (P = 0.031,0.029). The numbers of CECs and aCECs in SD and PD patients were higher than those before chemotherapy (P = 0.024,0.014; P = 0.036,0.028) The CECs and aCECs levels in patients with different efficacy of lung cancer, the number of aCECs PD, SD were higher than CR + PR (P = 0.022,0.009). Conclusion: The combination of VEGF and CECs and aCECs can be used as a good predictor to evaluate the angiogenesis and prognosis of lung cancer.