日本栝楼(Trichosanthes kirilowii var.japonica)的无菌苗经土壤杆菌的直接培养,得到日本栝楼的发根。发根经无激素的MS+0.5g/l头孢氨塞培养基继代培养后,除去细菌,并转入到MS液体培养基中,在25℃,转速为100rpm下暗培养3周。收采干燥的发根培养物,以氯仿提取,提取物经硅胶柱层析纯化,得2种化合物Chondrillasterol(1)和bryonolic acid(2),根据1H- Aseptic shoots of Trichosanthes kirilowii var. Japonica were directly cultured with Agrobacterium and the hair roots of Japanese kennels were obtained. Hair root After subculture with hormone-free MS + 0.5 g / l cefotaxime medium, the bacteria were removed and transferred to MS liquid medium for 3 weeks in dark at 25 ° C at 100 rpm. The dried hairy root cultures were collected and extracted with chloroform. The extracts were purified by silica gel column chromatography to give two compounds Chondrillasterol (1) and bryonolic acid (2)