论文部分内容阅读
目的:研究莪术油的在大鼠体内的分布特点。方法:采取大鼠灌胃给药,运用毛细管气相色谱法以α-香附酮为内标物同时测定莪术油中指标性成分莪术醇、莪术二酮在大鼠各个组织中的药物浓度。结果:莪术醇在0.002628~0.2102mg.mL-1范围内呈现良好的线性关系,莪术二酮在0.0004552~0.03642mg.mL-1范围内呈现良好的线性关系,精密度、稳定性及融冻次数考察均符合要求,测定方法的平均绝对回收率莪术醇为75.8~79.8%,莪术二酮为82.9%~86%,内标物α-香附酮的平均绝对回收率为69.4%~74.3%;平均方法回收率醇为59.9%~62.9%,平均方法回收率莪术二酮为64.1%~64.7%,均符合有关要求。通过各组织中莪术醇、莪术二酮含量分析,各组织中均含有两种指标性成分。结论:脾中指标性成分含量较高,心脏中指标性成分含量较低。
Objective: To study the distribution of Curcuma oil in rats. Methods: The rats were given intragastric administration, and the contents of curcumol and zedroneidone in Curcuma phaeocais were determined by capillary gas chromatography with α-ketones as internal standard. Results: Curcumol showed a good linear relationship in the range of 0.002628 ~ 0.2102mg.mL-1, Curcuma dione showed a good linear relationship in the range of 0.0004552 ~ 0.03642mg.mL-1, the precision, stability and number of freeze-thaw cycles The average absolute recovery of curcumol was 75.8-79.8% and that of curdione was 82.9% -86%. The average absolute recovery of the internal standard α-ketoconazole was 69.4% -74.3%. The average recovery rate of alcohol was 59.9% ~ 62.9%, the average recovery rate of curdione was 64.1% ~ 64.7%, which met the relevant requirements. Through the organization of Curcuma alcohol, Curcuma dione content analysis, each organization contains two index components. Conclusion: The content of the index components in the spleen is high, and the content of the index components in the heart is low.