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目的:建立测定唾液中双黄连有效成分绿原酸、黄芩苷含量的高效液相色谱法。方法:色谱柱:ODS C18柱(250mm×4.6mm,5μm);流动相分别是:甲醇-磷酸盐缓冲液(20∶80,pH2.7),甲醇-水(55∶45,pH3.0);柱温:55,30℃;流速:1.0mL.min-1;检测波长:274nm。结果:绿原酸、黄芩苷在唾液中最低定量限分别为0.30,0.15mg.L-1;平均回收率为:93.9%~111.8%和101.3%~104.4%。结论:该方法适用于唾液样品中双黄连有效成分的HPLC分析,取样量小,灵敏度高,操作简便易行,为利用唾液开展双黄连的临床药动学研究提供了方法学基础。
Objective: To establish a HPLC method for the determination of chlorogenic acid and baicalin content in Shuanghuanglian in saliva. Method: Column: ODS C18 column (250mm×4.6mm, 5μm); Mobile phase: methanol-phosphate buffer (20:80, pH2.7), methanol-water (55:45, pH3.0) Column temperature: 55, 30°C; Flow rate: 1.0 mL.min-1; Detection wavelength: 274 nm. Results: The minimum quantitation limits of chlorogenic acid and baicalin in saliva were 0.30 and 0.15 mg.L-1, respectively. The average recoveries were 93.9%-111.8% and 101.3%-104.4%. Conclusion: This method is suitable for the HPLC analysis of the active components of Shuanghuanglian in saliva samples. The sample size is small, the sensitivity is high, and the operation is simple and easy. It provides a methodological basis for the clinical pharmacokinetic study of Shuanghuanglian using saliva.