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以ＤＥＮ制成实验性肝癌大鼠，同时连续１２周分别给予肝癌大鼠干扰素α－２ｂ（ＩＦＮ）、卡介苗（ＢＣＧ）或两者联用，对照组给予生理盐水。于第１２周和１６周分离培养各组动物肝枯否细胞（ＫＣ）和血单核细胞（ＭＣ），并分别与人肝癌细胞联合培养，用￣３Ｈ－ＴｄＲ掺入法测定ＫＣ和ＭＣ对肝癌细胞的抑增殖作用。分离培养第１８周和２２周对照组肝癌大鼠的ＫＣ经体外ＩＦＮ、ＢＣＧ或两者联用后，同上测定ＫＣ对肝癌细胞的抑增殖作用。结果显示：１．肝癌大鼠的ＫＣ对肝癌细胞增殖仍具有一定的自然抑制作用，ＫＣ的作用明显强于ＭＣ；２．上述免疫刺激剂的在体应用或体外对ＫＣ的直接作用，均以ＩＦＮ和ＢＣＧ联用增强ＫＣ的抑肝癌细胞作用最强，最强者可增强５倍以上；３．单用免疫刺激剂者，ＢＣＧ的效果较ＩＦＮ强。讨论分析了联用免疫刺激剂增强ＫＣ抗肝癌最佳效果的机制及其实用意义。
Rats with hepatocellular carcinoma were established with DEN and simultaneously given 12 consecutive weeks of hepatoma rats with interferon α-2b (IFN), Bacillus Calmette-Guerin (BCG), or a combination of the two, and the control group was given physiological saline. The liver Kupffer cells (KC) and blood mononuclear cells (MC) of each group were isolated and cultured at the 12th and 16th weeks, and they were co-cultured with human hepatoma cells, and the KC and MC pairs were measured by ̄3H-TdR incorporation method. Inhibition of proliferation of hepatoma cells. After the 18th and 22th weeks of isolation and culture, the KC of the control group of hepatocarcinoma rats was combined with IFN, BCG or both in vitro to determine the effect of KC on the inhibition of hepatoma cells. The results show: 1. The KC of liver cancer rats still has certain natural inhibitory effect on the proliferation of hepatocellular carcinoma cells, and the effect of KC is obviously stronger than that of MC.2. The in vivo application of the above immunostimulating agents or the direct effect on KC in vitro was enhanced by the combination of IFN and BCG. The effect of KC on the inhibition of hepatoma cells was the strongest, and the strongest could be enhanced by more than 5 times. With the use of immunostimulants alone, the effect of BCG is stronger than that of IFN. Discussed and analyzed the mechanism of combined use of immune stimulators to enhance KC’s anti-hepatocellular carcinoma and its practical significance.