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目的 了解我省不同临床阶段HIV感染者体内病毒复制水平、机体免疫状况及其变化 ,探讨其与临床表现和预后之间的关系。方法 定期随访我省 1992～ 1999年发现的部分无症状HIV感染者和有艾滋病相关综合征 /艾滋病的病人 15例。应用核酸序列扩增技术 (NASBA)和花环形成试验分别测定不同临床阶段的HIV感染者血浆HIV - 1RNA拷贝数及外周血CD+4 和CD+8淋巴细胞的百分率。结果 8例HIV感染者的 10份血浆标本全部检出HIV - 1RNA ,而且有临床症状者的病毒载量明显高于无症状者 ,他们分别为 5 0 1± 0 2 9和 3 6 2± 0 2 (lg/ml)。病毒载量较高的感染者相应的CD+4 淋巴细胞百分率较低 ,有临床症状的感染者其CD+4 百分率多在 14%左右。结论 本实验提示我省发现的HIV - 1感染者体内普遍存在活跃的病毒复制 ,其病毒载量、CD+4 %、CD+4 /CD+8与临床表现、预后有关 ,鉴于本研究观察的例数有限 ,它们之间的关系有待进一步明确
Objective To understand the level of virus replication, the immune status and its changes in HIV-infected persons in different clinical stages in our province and to explore its relationship with clinical manifestations and prognosis. Methods 15 cases of asymptomatic HIV-infected persons and AIDS-related syndrome / AIDS patients found in our province from 1992 to 1999 were regularly followed up. The plasma HIV - 1 RNA copy number and the percentage of peripheral blood CD + 4 and CD + 8 lymphocytes in HIV - infected patients at different clinical stages were determined by using nucleic acid sequence amplification (NASBA) and rosette formation assays. Results HIV - 1 RNA was detected in 10 plasma samples from 8 HIV - infected patients and the viral load of clinical symptoms was significantly higher than that of asymptomatic patients (5 0 1 ± 0 2 9 and 3 6 2 ± 0 2 (lg / ml). The percentage of CD + 4 lymphocytes corresponding to those with higher viral load was lower, and the percentage of CD + 4 patients with clinical symptoms was mostly about 14%. Conclusions This study suggests that there is widespread viral replication in HIV - 1 infected people in our province. The viral load, CD + 4%, CD + 4 / CD + 8 are related to clinical manifestations and prognosis. The number of cases is limited, and the relationship between them needs to be further clarified