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目的本研究旨在研究JAK/STAT途径的负调控因子,细胞因子信号抑制因子1(SOCS-1)在宫颈癌中的作用。方法收集60份宫颈癌新鲜手术标本和20份非肿瘤标本,用real-time RT-PCR、Western blot和免疫组化实验分析宫颈癌不同病理阶段SOCS-1 mRNA和蛋白的表达、高危HPV感染的标本和无HPV感染的SOCS-1的表达,并与正常宫颈组织进行比较。结果与正常组织中SOCS-1比较,58%的肿瘤组织不表达或减少表达,免疫组化检测、realtime RT-PCR和Western blot的结果一致。与未感染HPV的标本比较,高危HPV感染的标本低表达或不表达SOCS-1。结论高危型HPV16、HPV18的感染可导致SOCS-1的转录失活,表明高危型HPV癌蛋白抑制SOCS-1的表达,意味着SOCS-1在宫颈癌发展中起重要作用。
Objective This study aimed to investigate the role of negative regulator of JAK / STAT pathway and cytokine signaling inhibitor 1 (SOCS-1) in cervical cancer. Methods Sixty fresh specimens of cervical cancer and 20 non-tumor specimens were collected. The expression of SOCS-1 mRNA and protein in different pathological stages of cervical cancer was analyzed by real-time RT-PCR, Western blot and immunohistochemistry. Specimens and HPV-infected SOCS-1 expression were compared with normal cervical tissue. Results Compared with SOCS-1 in normal tissues, 58% of tumor tissues did not express or reduce expression. The results of immunohistochemistry, realtime RT-PCR and Western blot were the same. Compared with non-HPV-infected specimens, high-risk HPV-infected specimens showed low or no expression of SOCS-1. Conclusion The infection of high-risk HPV16 and HPV18 can lead to the transcriptional inactivation of SOCS-1, indicating that high-risk HPV oncoprotein inhibits the expression of SOCS-1, suggesting that SOCS-1 plays an important role in the development of cervical cancer.