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目的:研究青海世居汉族人群乳腺癌易感性与X射线损伤修复交叉互补基因1(XRCC1)C26304T位点基因多态性的相关关系。方法:应用聚合酶链反应-限制性片段多态性(PCR-RFLPs)方法检测青海世居汉族人群30例乳腺癌患者(乳腺癌组)及30例乳腺良性病变患者(对照组)XRCC1C26304T基因多态性分布情况。同时运用统计学方法对XRCC1 C26304T的基因型和基因频率进行分析。结果:XRCC1 C26304T基因CC、CT、TT基因型在对照组中分别是56.7%、33.3%、10%,而在乳腺癌组中分别是50.0%、36.7%、13.3%。TT基因型在对照组及乳腺癌组中,相对于CT、CC基因组表达较低,但是对照组和乳腺癌组间并没有明显差异(P>0.05)。经过分析,C、T基因频率在对照组中是0.733、0.267,而在乳腺癌组中是0.683、0.317,两组比较,C、T基因频率也无明显差异(P>0.05)。结论:XRCC1 C26304T基因多态性可能与青海世居汉族人群乳腺癌易感性无关。
OBJECTIVE: To study the correlation between the susceptibility of breast cancer and the polymorphism of XRCC1 C26304T in X-ray repair cross-complementing gene 1 (XRCC1) in Qinghai Shiju Han population. METHODS: Polymerase chain reaction-restriction fragment polymorphism (PCR-RFLPs) was used to detect 30 XRCC1C26304T genes in 30 breast cancer patients (breast cancer group) and 30 benign breast disease patients (control group) in the Shiju Han population in Qinghai Province. State distribution. At the same time, statistical methods were used to analyze the genotype and gene frequency of XRCC1 C26304T. RESULTS: The XRCC1 C26304T gene CC, CT, TT genotypes were 56.7%, 33.3%, and 10% in the control group, and 50.0%, 36.7%, and 13.3% in the breast cancer group, respectively. The TT genotype was lower in the control and breast cancer groups than in the CT and CC genomes, but there was no significant difference between the control and breast cancer groups (P>0.05). After analysis, the frequencies of C and T genes were 0.733 and 0.267 in the control group, and 0.683 and 0.317 in the breast cancer group. There was no significant difference in the frequency of C and T genes between the two groups (P>0.05). Conclusion: The XRCC1 C26304T gene polymorphism may not be related to the susceptibility to breast cancer in the Shiju Han population in Qinghai.