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通过研究富氢水(Hydrogen-rich water)对Wistar雄性大鼠肠道菌群的影响,建立了富氢水和肠道微生态之间的关联,并研究了富氢水对模拟失重大鼠的肠道微生态调节作用,为预防航天员肠道微生态的失调和促进飞行后肠道微生态平衡的恢复提供依据。研究通过构建含有待测细菌的16S rRNA基因序列的重组质粒作为标准品,利用SYBR GreenⅠ实时荧光定量PCR的方法,检测富氢水对大鼠盲肠和结肠内容物中5种常见细菌的影响。试验成功构建了5种肠道菌的标准曲线,并实现了对肠内容物中5种细菌的定量。结果显示,模拟失重使肠道中肠球菌和大肠杆菌的拷贝数上升,产气荚膜梭菌的拷贝数下降,而富氢水对肠球菌则有明显的抑制作用。
By studying the effects of hydrogen-rich water on intestinal flora in Wistar male rats, the correlation between hydrogen-rich water and gut microecology was established and the effect of hydrogen-rich water on Intestinal microflora regulation, to prevent astronauts intestinal micro-ecological disorders and to promote the recovery of post-flight intestinal micro-ecological balance provide the basis. In this study, the effect of hydrogen-rich water on the five common bacteria in the cecal and colon contents of rats was tested by constructing a recombinant plasmid containing the 16S rRNA gene sequence of the bacterium to be tested as a standard by SYBR Green I real-time fluorescence quantitative PCR. The test successfully constructed a standard curve of five kinds of intestinal bacteria and achieved the quantification of five kinds of bacteria in the intestinal contents. The results showed that simulated weightlessness increased the number of enteric enterococci and Escherichia coli copies, the copy number of Clostridium perfringens decreased, while the hydrogen-rich water had a significant inhibitory effect on enterococci.