目的探讨人类冻融胚胎质量与临床妊娠结局的关系。方法回顾性分析了738例进行玻璃化冷冻的人类冻融胚胎移植(FET)患者的相关资料,根据移植优质胚胎的数量、卵裂球损伤的程度、移植胚胎中是否含8细胞胚胎以及含8细胞胚胎的数目,将其分为4组,分别比较各组间的临床妊娠率和多胎率之间的差异。结果 1移植2枚优质胚胎组临床妊娠率均高于移植0枚优质胚胎组和移植1枚优质胚胎组(P<0.05),并且与移植3枚优质胚胎组基本相同;各组间的多胎率差异无统计学意义;2移植胚胎完整组、损伤组、损伤混合组各组间的临床妊娠率、多胎率差异均无统计学意义;3移植8细胞胚胎组的临床妊娠率显著高于移植6~7或9~13细胞胚胎组(P<0.05),且多胎率差异无统计学意义。4移植2枚8细胞胚胎组的种植率、临床妊娠率显著高于移植0枚8细胞胚胎组和移植1枚8细胞胚胎组(P<0.05);移植3枚8细胞胚胎组的多胎率显著高于移植0枚8细胞胚胎组(P<0.05),其余组间差异无统计学意义。结论 FET中移植的优质胚胎数目和8细胞胚胎数目是影响临床妊娠率和多胎率的重要因素;胚胎损伤对临床妊娠率和多胎率无明显影响。因此,FET时移植至少1枚优质胚胎或1枚8细胞胚胎,既可以保证其临床妊娠率,同时又可以降低多胎率。 Objective To investigate the relationship between human frozen-thawed embryo quality and clinical pregnancy outcome. Methods The data of 738 human frozen-thawed embryo transfer (FET) patients who underwent vitrification were retrospectively analyzed. According to the number of high quality embryos transferred, the extent of blastomere damage, whether the embryos contained 8-cell embryos and those containing 8 The number of cell embryos was divided into 4 groups, and the differences between clinical pregnancy rates and multiple birth rates were compared among groups. Results 1 The clinical pregnancy rate of two high quality embryo transplantation groups was higher than that of 0 high quality embryo transplantation group and 1 high quality embryo transplantation group (P <0.05), and was similar to that of 3 high quality embryo transplantation groups. The multiple pregnancy rate There was no significant difference between the two groups. (2) There was no significant difference in clinical pregnancy rate and multiple birth rate among the groups of intact embryo transfer, injury and mixed injury groups. 3 The clinical pregnancy rate of the 8-cell embryo transplantation group was significantly higher than that of transplantation 6 ~ 7 or 9 ~ 13 cell embryos group (P <0.05), and there was no significant difference in multiple birth rate. 4 transplanted two 8-cell embryos group, the clinical pregnancy rate was significantly higher than that of the transplantation of 0 8-cell embryo group and the transplantation of 1 8-cell embryo group (P <0.05); the transplanted 3 8-cell embryos group had significant multiple pregnancy rate Higher than transplanted 0 8-cell embryos group (P <0.05), the difference between the other groups was not statistically significant. Conclusion The number of high-quality embryos and the number of 8-cell embryos transplanted in FET are important factors affecting clinical pregnancy rate and multiple birth rate. Embryonic injury has no significant effect on clinical pregnancy rate and multiple birth rate. Therefore, FET transplantation of at least one high-quality embryo or an 8-cell embryo, both to ensure its clinical pregnancy rate, while reducing the rate of multiple births.