以咔唑为起始原料,通过硝化、还原、酰化合成了3,6-二(3-丙酰氯氨基)咔唑,再分别与4个含氮化合物进行烷基化反应,合成了4个具有不同取代基的3,6-二氨基咔唑衍生物,用1HNMR、13CNMR和ESI-MS进行了结构表征。通过UV-Vis谱、荧光光谱、圆二色谱和热变性实验研究了合成化合物与小牛胸腺(Ct)-DNA的结合能力。结果表明,化合物Ⅳa及Ⅳb作为DNA嵌入剂与Ct-DNA有较好的结合能力。 Using carbazole as the starting material, 3,6-bis (3-propionylchloroamino) carbazole was synthesized by nitration, reduction and acylation, and then alkylated with four nitrogenous compounds respectively to synthesize 4 The 3,6-diamino carbazole derivatives with different substituents were characterized by 1H NMR, 13CNMR and ESI-MS. The binding ability of the synthesized compounds to calf thymus (Ct) -DNA was studied by UV-Vis spectroscopy, fluorescence spectroscopy, circular dichroism and thermal denaturation experiments. The results showed that the compounds Ⅳa and Ⅳb as DNA intercalator and Ct-DNA have better binding ability.