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采用高效液相色谱法测定样品中丹皮酚含量,以SpherisobC18为固定相,流动相为甲醇05%磷酸(60∶40),检测波长274nm,线性范围为005~05μg,平均回收率9928%,RSD为125%。
The content of paeonol in the sample was determined by HPLC. Spherisob C18 was used as the stationary phase. The mobile phase was methanol 0.5% phosphoric acid (60:40), the detection wavelength was 274 nm and the linear range was 005 ~ 05 μg , The average recovery rate was 9928% and RSD was 125%.