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目的：探讨急性早幼粒细胞白血病（ＡＰＬ）发病初期和维甲酸（ＡＴＲＡ）及三氧化二砷（Ａｓ２Ｏ３）治疗期间组织因子（ＴＦ）的表达。方法：采用ＥＬＩＳＡ法测定血浆可溶性纤维蛋白单体复合物（ＳＦＭＣ）、Ｄ二聚体（ＤＤ）和细胞裂解液ＴＦ含量；ＲＴＰＣＲ法测定细胞ＴＦｍＲＮＡ的转录情况。结果：发病时，患者血浆ＳＦＭＣ和ＤＤ水平、骨髓分离的单个核细胞悬液的促凝活性（ＰＣＡ）、细胞裂解液的ＴＦ水平、ＴＦｍＲＮＡ的转录均显著升高，经ＡＴＲＡ和Ａｓ２Ｏ３治疗后显著降低。结论：ＡＴＲＡ和Ａｓ２Ｏ３都能抑制ＴＦｍＲＮＡ的转录，降低ＡＰＬ细胞的ＴＦ和ＰＣＡ，抑制凝血亢进和继发纤溶，从而在治疗早期即可减轻患者的出血症状。
Objective: To investigate the expression of tissue factor (TF) during the early onset of acute promyelocytic leukemia (APL) and during the treatment with ATRA and As2O3. METHODS: Plasma soluble fibrin monomer complex (SFMC), D-dimer (D-D) and TF content in cell lysate were determined by ELISA. The transcription of TFmRNA was determined by RT-PCR. Results: At the time of onset, plasma SFMC and D-D levels, pro-coagulant activity (PCA) of bone marrow isolated mononuclear cell suspensions, TF levels in cell lysates, transcription of TF mRNA were significantly increased, and were treated with ATRA and As2O3. After significantly reduced. Conclusion: Both ATRA and As2O3 can inhibit the transcription of TF mRNA, decrease the TF and PCA of APL cells, and inhibit the hypercoagulability and secondary fibrinolysis, which can reduce the bleeding symptoms in the early stage of treatment.