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目的:建立银翘散(YQS)不同复方配伍组及君(J),臣(C),佐(Z),使(S)关系的HPLC指纹图谱,找出配伍前后物质基础变化及复方配伍过程中物质基础相互作用关系,从整体上探讨YQS复方配伍关系。方法:利用Ulti Mate 3000 XRS型超快速液相色谱仪,Thermo C18色谱柱(4.6 mm×250 mm,5μm),流动相甲醇-0.05%磷酸水梯度洗脱,检测波长237 nm,流速1 m L·min-1,对YQS及君臣佐(J,C,Z),君臣使(J,C,S),君佐使(J,Z,S),君臣(J,C),J,C,Z,S不同配伍关系进行HPLC指纹图谱研究;利用指纹图谱处理软件对各组间HPLC指纹图谱进行数据分析。结果:YQS与各配伍组相似度由高到低为J,C,S(0.981)>J,C(0.959)>J,C,Z(0.953)>J,Z,S(0.806);YQS与J,C,Z,S组相似度由高到低为J(0.749)>C(0.573)>Z(0.136)>S(0.104)。YQS配伍合煎与单煎相加后的指纹图谱相比,14个峰面积降低,63个峰面积增加。结论:君臣药是YQS物质基础主要来源;本文从物质基础组成的角度,对YQS中J,C,Z,S在全方中的重要性进行了数据分析,使传统方解更具有说服力。
OBJECTIVE: To establish the HPLC fingerprints of different YQS compatibility groups, Jun (C), Z (Z) and (S) compatibility groups to find out the changes of material basis and compatibility of compound before and after compatibility In the material basis of the interaction between the YQS compound to discuss the relationship between the overall. Methods: The Ulti Mate 3000 XRS supercritical liquid chromatograph was used on a Thermo C18 column (4.6 mm × 250 mm, 5 μm). The mobile phase was eluted with methanol-0.05% phosphoric acid. The detection wavelength was 237 nm and the flow rate was 1 m L · Min-1, and YJS and Junchenzuo (J, C, Z) and Jun-min J, C and S, J, Z and S, Z, S different compatibility relations for the HPLC fingerprinting study; the use of fingerprinting software for each group of HPLC fingerprinting data analysis. Results: The similarity between YQS and each compatibility group was J, C and S (0.981)> J, C (0.959)> J, C and Z (0.953)> J and Z and S The similarity of J, C, Z and S groups was J (0.749)> C (0.573)> Z (0.136)> S (0.104). Compared with the fingerprinting of YQS, the peak area of 14 peaks and the area of 63 peaks increased. Conclusion: Monarch drug is the main source of YQS material basis. From the perspective of material basic composition, this paper analyzes the importance of J, C, Z, S in all aspects of YQS, making the traditional square solution more convincing.