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为寻求一种灵敏、经济、快捷检测辣椒轻斑驳病毒(Pepper mild mottle Tobamovirus,PMMoV)的检测方法,以辣椒轻斑驳病毒侵染的甜椒叶片为材料,采用DIG标记的核酸斑点杂交、RT-PCR和DAS-ELISA3种方法检测不同系列稀释倍数的辣椒叶片总RNA和叶片提取液,测试3种方法的稀释限点。结果表明,RT-PCR灵敏度最高,地高辛斑点杂交灵敏度次之,DAS-ELISA灵敏度最低。在3种方法中地高辛斑点杂交方法与其他两种方法相比灵敏度较高,不需要任何特定仪器,而且适合大量样品的检测,所以此方法有很好的应用前景。
In order to find out a sensitive, economical and rapid detection method of pepper mottle Tobamovirus (PMMoV), pepper seeds infected by pepper light mottle virus were used as materials and DIG-labeled nucleic acid dot blot hybridization and RT- PCR and DAS-ELISA three kinds of methods to detect different series of dilutions of total RNA and leaf extract of pepper leaves, the three methods of dilution limit test. The results showed that the sensitivity of RT-PCR was the highest, the sensitivity of digoxin blot hybridization was the second, and the sensitivity of DAS-ELISA was the lowest. Compared with the other two methods, the digoxin hybridization method is more sensitive than the other two methods and does not require any special instrument and is suitable for the detection of a large number of samples. Therefore, this method has a good application prospect.