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近几年来,国内外在十余种植物上发现由离体细胞再生的植株中高频率地出现变异体,这被称为体细胞无性系变异(Somaclonal variation)。1967年,Murashige 和 Nakano 首先较明确地指出组织培养再生植株中出现遗传性变异。继而 Heinz 等(1969、1977)和 D’Amato(1978)较详细地研究了再生植株染色体水平上的变异。Widholm(1977)、Chaleff 和 Parsons(1978)、Lauyer 等(1980)、Sung 和 Jacques(1980)等利用组织培养物的变异现象选出了一些基因水平上的变异体,包括抗氨基酸类似物突变体,抗碱基类似物突变体,抗抗生素和抗
In recent years, at home and abroad more than ten kinds of plants found in plants regenerated from ex vivo cells in high frequency variants, which is known as somaclonal variation (Somaclonal variation). In 1967, Murashige and Nakano first pointed out more clearly that there was a genetic variation in the tissue culture of regenerated plants. Subsequently, Heinz et al. (1969, 1977) and D’Amato (1978) studied the variation of chromosomal levels in regenerated plants in more detail. Some variants at the gene level were selected using the phenomenon of variation in tissue culture by Widholm (1977), Chaleff and Parsons (1978), Lauyer et al. (1980), Sung and Jacques (1980), including mutants resistant to amino acid analogues , Anti-base analog mutants, antibiotics and anti-anti