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大豆遗传转化的受体主要有未成熟子叶、胚性悬浮培养物(细胞团)、胚轴、子叶节和子房等。其中,大豆未成熟子叶和胚性悬浮培养物主要作为基因枪法的转化受体,由于它们的体积较小,作为基因枪轰击的靶较为经济;大豆胚轴和子叶节可以作为农杆菌介导法的主要转化受体;子房可作为花粉管通道法的转化受体。1基因枪法基因枪法又称生物弹法或微粒枪法、微粒轰击法,是依赖高速度的金属微粒将外源基因引入活细胞的一种
Soybean genetic transformation of the main receptor immature cotyledons, embryogenic suspension cultures (cell mass), hypocotyls, cotyledonary node and ovary and so on. Among them, immature soybean cotyledons and embryogenic suspension cultures are mainly used as transformation acceptors for the gene gun method. Because of their smaller size, they are more economical targets for gene gun bombardment. Soybean hypocotyl and cotyledonary node can be used as Agrobacterium-mediated methods Of the major transformation receptors; ovary can be used as a pollen tube channel receptor conversion. A gene gun law Bi gun method, also known as bio-bomb method or particle gun method, particle bombardment method is dependent on high-speed metal particles introduced foreign genes into a living cell