目的：构建乙型肝炎病毒基因疫苗 ｐ Ｃ Ｒ３．１ Ｓ，观察重组人白细胞介素２（ｒｈ Ｉ Ｌ２）作为佐剂对其诱导 Ｂ Ａ Ｌ Ｂ／ｃ 小鼠产生免疫应答的影响． 方法：以 Ｅ Ｌ Ｉ Ｓ Ａ 法检测免疫小鼠血清抗 Ｈ Ｂｓ，另用３ Ｈ Ｔｄ Ｒ 参入法测定淋巴细胞增殖活性，初步研究不同免疫组的体液和细胞免疫应答． 结果：ｒｈ Ｉ Ｌ２ 组免疫小鼠抗 Ｈ Ｂｓ 抗体和淋巴细胞增殖活性与对照组相比有显著差异（ Ｐ＜ ０．０５）． 结论： Ｈ Ｂ Ｖ 基因疫苗可诱发 Ｂ Ａ Ｌ Ｂ／ｃ 产生良好的免疫应答，ｒｈ Ｉ Ｌ２ 作为佐剂可增强其免疫效应 Objective: To construct hepatitis B virus gene vaccine pC R3.1S, and to observe the effect of recombinant human interleukin-2 (IL-2) as an adjuvant on the immune response induced by B A L B / c mice . Methods: Serum anti-H Bs of mice were detected by E L I S A method. Lymphocyte proliferative activity was measured by 3H-Td R incorporation method. The humoral and cellular immune responses of different immune groups were preliminarily studied. Results: The anti-H Bs antibody and lymphocyte proliferative activity of rh I L2 immunized mice were significantly different from those of the control group (P <0.05). Conclusion: H B V gene vaccine can induce B A L B / c to produce a good immune response, rh I L 2 as an adjuvant can enhance its immune response