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目的研究微血管密度在胃癌癌变多阶段组织中的表达及其意义。方法标本取自中山大学中山医学院病理学教研室保存的2000年6月至2004年2月的病理组织蜡块,其中慢性胃炎56例、慢性萎缩性胃炎50例、肠化生59例、不典型增生61例;在胃癌组织标本中,无淋巴结转移胃癌组织55例,有淋巴结转移胃癌组织57例。免疫组织化学Envision法显示胃癌癌变多阶段组织中CD34的表达。结果Envision方法检测微血管密度的结果(个/高倍视野,用x-±s表示):慢性胃炎组为13±10;慢性萎缩性胃炎组为11±7;肠化生组为13±9;不典型增生组为17±11;无淋巴转移胃癌组为27±11;有淋巴转移胃癌组为28±10。微血管密度在慢性胃炎组、萎缩性胃炎组、肠化生组组间比较,差异无统计学意义(均P>0·05);萎缩性胃炎组、肠化生组、不典型增生组组间比较,差异无统计学意义(均P>0·05);慢性胃炎组低于不典型增生组(P<0·05);慢性胃炎、萎缩性胃炎、肠化生、不典型增生各组均低于无淋巴转移胃癌组织、有淋巴转移胃癌组(均P<0·05);在胃癌组中,无淋巴转移胃癌组织与有淋巴转移胃癌组织组间比较,差异无统计学意义(P>0·05),其组织学病理程度高中分化组、与低分化组间差异也无统计学意义(P>0·05)。结论微血管密度在胃癌癌变多阶段组织中增加与胃癌的发生有关,与淋巴结转移无关。
Objective To study the expression and significance of microvessel density in multi-stage gastric cancer tissue. Methods The specimens were collected from the pathological tissue paraffin blocks from June 2000 to February 2004 in the Department of Pathology of Zhongshan Medical College of Sun Yat-sen University. Among them, there were 56 chronic gastritis, 50 chronic atrophic gastritis, 59 intestinal metaplasia, 61 cases of hyperplasia; in gastric cancer tissue samples, 55 cases of gastric cancer without lymph node metastasis, 57 cases of gastric cancer with lymph node metastasis. Immunohistochemistry Envision method shows the expression of CD34 in multi-stage tissue of gastric cancer. Results Envision method to detect the results of microvessel density (a / high power field, with x- ± s): chronic gastritis was 13 ± 10; chronic atrophic gastritis was 11 ± 7; intestinal metaplasia was 13 ± 9; The typical hyperplasia group was 17 ± 11; the group without lymph node metastasis was 27 ± 11; the group with lymph node metastasis was 28 ± 10. Microvessel density in chronic gastritis group, atrophic gastritis group, intestinal metaplasia group comparison, the difference was not statistically significant (all P> 0.05); atrophic gastritis group, intestinal metaplasia group, atypical hyperplasia group (P> 0.05), chronic gastritis group was lower than the atypical hyperplasia group (P <0.05), chronic gastritis, atrophic gastritis, intestinal metaplasia, atypical hyperplasia in each group (P <0.05). There was no significant difference between gastric cancer group and non-lymph node metastasis gastric cancer group and lymph node metastasis gastric cancer group (P> 0.05). There was also no significant difference between the high differentiation group and the poorly differentiated group (P> 0.05). Conclusions The increase of microvessel density in multi-stage cancerous tissues of gastric cancer is related to the occurrence of gastric cancer, but not to lymph node metastasis.