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目的:研究四妙君逸软膏的镇痛作用及其镇痛作用与阿片受体、β-内源性阿片肽(β-EP)、5-羟色胺(5-HT)的关系。方法:采用小鼠热板法、扭体法观察四妙君逸软膏对小鼠镇痛作用的影响;采用纳洛酮拮抗实验,醋酸致痛后检测小鼠血清β-EP和脑组织5-HT的含量,探讨四妙君逸软膏的镇痛作用机制。结果:四妙君逸软膏能够明显延长痛阈和抑制小鼠扭体反应,镇痛作用优于马应龙麝香痔疮膏。其镇痛作用能被纳洛酮部分拮抗,并能明显提高小鼠血清β-EP和脑组织5-HT的含量。结论:四妙君逸软膏能够提高小鼠血清β-EP和脑组织5-HT的含量,具有明确的镇痛作用。其镇痛作用与阿片受体、β-EP、5-HT有关。
Objective: To study the analgesic effect and analgesic effect of Si Miao Junyi Ointment on the relationship between opioid receptor, β-endorphin (β-EP) and 5-hydroxytryptamine (5-HT). Methods: Mouse hot plate method and writhing method were used to observe the effect of Si Miao Jun Yi ointment on the analgesic effect of mice. Naloxone antagonistic experiment and acetic acid induced pain were used to detect the serum levels of β-EP and brain 5- HT content, to explore the analgesic mechanism of Si Miao Jun Yi ointment. Results: Si Miao Jun Yi ointment can significantly extend the pain threshold and inhibit writhing reaction in mice, analgesic effect is better than Ma Ying Long musk hemorrhoids ointment. Its analgesic effect can be partially antagonized by naloxone, and can significantly increase the level of serum β-EP and brain tissue 5-HT in mice. Conclusion: Si Miao Junyi ointment can increase serum β-EP and brain tissue 5-HT content, with a clear analgesic effect. Its analgesic effect and opioid receptors, β-EP, 5-HT.