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利用AFLP技术对94份野生桃种质资源进行了DNA多态性分析,从64对E+2/M+3引物组合中筛选出9对引物用于扩增基因组DNA,共获得清晰可辨的236个标记,其中多态性标记为141个,多态性检出率为59.9%。UPGMA聚类结果显示,在遗传距离约为0.3267时,普通桃、陕甘山桃、光核桃3个种被聚为一类,与这3个种相近的为新疆桃,其次为甘肃桃,最远为山桃(白花山桃、红花山桃)。在遗传距离0.2178处,五月鲜扁干、白黏胡桃和珲春桃3份材料各自聚为一类。在遗传距离约0.1997处,普通桃又可明显分为4个组:组I包括原产于南方和西北地区的地方品种,且大部分南方蟠桃主要聚在此类;组II仅有油桃(甘肃酒泉)一个野生材料;组III包括野生毛桃和大部分原产于北方的地方品种,北方硬肉桃主要聚在这一类;组IV由白碧桃和白花山碧桃2个观赏桃品种组成。
Ninety-four accessions of wild peach germplasm were analyzed for DNA polymorphism using AFLP. Ninety-four pairs of primer combinations were screened from 64 pairs of E + 2 / M + 3 primers for genomic DNA amplification to obtain a clear and legible 236 markers, of which 141 were polymorphic markers, the rate of polymorphism was 59.9%. The results of UPGMA clustering showed that the three species of common peach, Shanxi Ganshan peach and light walnut were clustered into one category at the genetic distance of 0.3267, which was similar to the three peaches, followed by Gansu peach and farthest For the peach (white peach, safflower Peach). At the genetic distance of 0.2178, the fresh materials of May, white sticky peach and Hunchun were all clustered together. At the genetic distance of about 0.1997, the common peach was apparently divided into four groups: Group I included local cultivars native to the South and Northwest China, and most of the southern Peach mainly clustered in this category; Group II was nectarine Gansu Jiuquan), a wild material; Group III, including wild peach and most of the local varieties native to the north, the northern hard peach mainly in this category; group IV from the white peach and white Huashan Pitt 2 ornamental peach varieties.