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植物水通道蛋白PIP不仅担负细胞间或细胞内外水分子输导的基本功能,还参与植物-微生物互作与植物防卫反应,这种双重功能的调控机制目前还不清楚。水稻OsPIP1;2和拟南芥AtPIP1;4可以与水稻黄单胞III型泌出蛋白Hpa1互作,Hpa1定位于植物细胞的质外体,诱导过氧化氢在质外体产生及向原生质转运,进而影响植物防卫反应与对病原细菌的抗性。根据植物水通道蛋白拓扑结构与病原细菌Ⅲ型分泌系统工作模型,水稻OsPIP1;2与Hpa1互作的功能域是互作发生的分子基础。互作引发信号转导,调控过氧化氢信号从植物细胞的质外体向原生质转运与植物防卫反应。由于Hpa1对Ⅲ效应蛋白来说具有转位子的功能特征,OsPIP1;2-Hpa1还可能对水稻黄单胞菌Ⅲ型效应蛋白从细菌细胞向植物细胞转运发生调控作用。围绕这些设想进行研究,可以深入阐释水稻-黄单胞菌互作机制,同时为植物水通道蛋白功能调控提供新的见解。
The plant aquaporin PIP not only serves as a basic function of water molecules transport between cells or inside and outside the cell, but also participates in plant-microorganism interaction and plant defense response. The regulatory mechanism of this dual function is unclear. Rice OsPIP1; 2 and Arabidopsis AtPIP1; 4 can interact with Hpa1, a type of cytochrome III excretion protein in rice. Hpa1 is localized in the apoplast of plant cells and induces the production and transport of hydrogen peroxide to apoplast. Thus affecting plant defense response and resistance to pathogenic bacteria. According to the aquaporin topology of plants and the model of pathogenic bacteria type Ⅲ secretion system, the functional domain of OsPIP1; 2 interacting with Hpa1 is the molecular basis of interaction. Interaction leads to signal transduction that regulates the transport of hydrogen peroxide signals from plant cells to the protoplasm and plant defense responses. OsPIP1; 2-Hpa1 may also play a role in regulating the transport of X-type effect protein from the bacterial cell to the plant cell due to Hpa1 having the transposon function to the III-responsive protein. Based on these assumptions, it is possible to further elucidate the mechanism of interaction between the two genes and to provide new insight into the regulation of aquaporin function in plants.