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以分布于棉花26条染色体的36对SSR核心引物,在6份成套中棉所系列杂交种间筛选双亲互补型杂合位点作为纯度检测标记,采用单位点平均法与双位点差异法统计SSR标记检测结果;在棉铃期考察棉花株型、铃形、叶形、花及茎等性状,比对田间表型与分子检测结果,分析相关性。结果表明,34对核心引物在6个杂交种上获得101个杂合位点,平均每个杂交种具有16.8个。田间表型鉴定结果高于分子标记检测结果,单位点平均法的相关性高于双位点差异法,校正后的相关性进一步提高,相关系数r=0.7841,呈高度相关。EST-SSR标记的检测结果与田间表型鉴定结果的相关性显著高于Genomic-SSR标记。相同染色体上的不同引物检测结果差异较小。不同的统计方法与标记类型获得的分子标记检测结果差异较大。高度纯合的亲本将会有效提高分子标记鉴定结果与表型鉴定结果的相关性。
36 pairs of SSR core primers distributed on 26 chromosomes of cotton were used to screen the parents’ complementary heterozygous loci as the markers of purity detection in 6 sets of cotton germplasm hybrids. The single-point average method and double-locus difference method SSR markers. The cotton plant type, bell shape, leaf shape, flower shape and stem shape were investigated in cotton boll stage. The correlation between phenotype and molecular test results was analyzed. The results showed that 34 pairs of core primers obtained 101 heterozygous loci on 6 hybrids with an average of 16.8 per hybrid. The field phenotype identification results were higher than the molecular marker test results. The correlation of single point average method was higher than that of the two-point difference method, and the corrected correlation was further improved. The correlation coefficient r = 0.7841 was highly correlated. The correlation between the EST-SSR marker and the phenotype of the field phenotype was significantly higher than that of the Genomic-SSR marker. Different primers on the same chromosome showed little difference. Different statistical methods and labeling types obtained by the molecular marker test results vary greatly. Highly homozygous parents will effectively improve the correlation between molecular marker identification and phenotypic identification.