论文部分内容阅读
研究了以十六烷基三甲基溴化铵(或十二烷基硫酸钠)为准固定相的胶束电动毛细管色谱法中的双峰及其形成原因。指出在一定的条件下一种组分可以出现2个峰。实验表明,某一组分相应双峰的相对峰面积取决于该组分与表面活性剂之间的反应时间与温度以及样品中表面活性剂的浓度。当样品中表面活性剂浓度增加时,双峰中一个峰的相对峰面积增加,而另一个减小;温度可以加速反应的过程。这意味着被分析物与表面活性剂之间的相互作用是一个慢过程,这种相互作用可以产生一种稳定的物质,并导致双峰的形成。十六烷基三甲基溴化铵与间羟基苯甲酸反应产物的红外与核磁共振光谱证实了这一点。
The bimodal and its formation in micellar electrokinetic capillary chromatography with cetyltrimethylammonium bromide (or sodium dodecyl sulfate) as the stationary phase was investigated. It is pointed out that under certain conditions, two peaks appear in one kind of component. Experiments show that the relative peak area of the corresponding bimodal peak of a component depends on the reaction time and temperature between the component and the surfactant and the concentration of the surfactant in the sample. As the surfactant concentration increases in the sample, the relative peak area of one peak in the bimodal increases while the other decreases; the temperature accelerates the reaction. This means that the interaction between the analyte and the surfactant is a slow process that produces a stable substance and results in the formation of bimodal peaks. This is confirmed by IR and NMR spectra of the reaction product of cetyltrimethylammonium bromide and m-hydroxybenzoic acid.