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目的 观察降钙素基因相关肽 (CGRP)对短暂性脑缺血脑组织神经元凋亡 /死亡的影响 ,探讨其对缺血脑组织的保护作用。方法 应用颈动脉负压分流法制作大鼠短暂性全脑缺血模型 ;于再灌注开始 ,经颈动脉注入CGRP ;应用末端脱氧核苷酸转移酶缺口标记 (TUNEL)法检测神经元凋亡 /死亡。结果 全脑缺血海马CAI区和皮层TUNEL阳性细胞百分率分别为 78.6 0± 4 .82和 5 4 .5 2± 5 .33,显著高于假手术组(2 .4 7± 0 .5 9和 3.4 0± 1.10 ) (P <0 .0 1) ;CGRP组海马和皮层神经细胞凋亡的百分率分别为 5 9.4 2± 3.71和36 .5 7± 5 .32 ,明显低于全脑缺血组 (P <0 .0 1)。结论 CGRP可减少大鼠短暂性全脑缺血脑组织神经元的凋亡 /死亡 ,对缺血神经元具有保护作用。
Objective To investigate the effect of calcitonin gene related peptide (CGRP) on neuronal apoptosis / death in transient ischemic brain tissue and its protective effect on ischemic brain tissue. Methods The model of transient global cerebral ischemia was established by carotid artery negative pressure shunt. At the beginning of reperfusion, CGRP was injected into the carotid artery. The apoptosis and apoptosis of neurons were detected by TUNEL. death. Results The percentages of TUNEL positive cells in hippocampal CA1 and cortex were 78.6 ± 4.82 and 54.2 ± 5.33, respectively, which were significantly higher than those in sham operation group (2.47 ± 0.59 and 3.4 ± 1.10) (P <0.01). The percentages of apoptotic neurons in hippocampus and cortex in CGRP group were 5 9.42 ± 3.71 and 36.57 ± 5.32, respectively, which were significantly lower than those in cerebral ischemia group (P <0. 01). Conclusion CGRP can reduce the apoptosis / death of neurons in transient global cerebral ischemia in rats, and has a protective effect on ischemic neurons.