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[目的]研究CDC6、Survivin在宫颈癌组织中的表达及其与HPV16/18感染的相关性,以探讨宫颈癌的发生机制,进一步寻找有利于宫颈癌诊断的新的分子标志物,以指导其诊断与预后。[方法]采用免疫组织化学方法对50例宫颈癌、20例宫颈上皮内瘤样病变CINⅠ、20例CINⅡ-Ⅲ、20例正常宫颈组织进行CDC6、Survivin的检测,同时采用PCR技术检测HPV16/18感染情况。[结果](1)在宫颈癌组织中CDC6和Survivin表达的阳性率高于CIN和正常宫颈组织,差异有统计学意义(均P﹤0.05),且CDC6和Survivin阳性表达率与病理分级、淋巴结转移有关,差异有统计学意义(均P﹤0.05),CDC6、Survivin阳性表达率均与年龄分组、临床分期无关(均P﹥0.05)。(2)HPV16/18在正常宫颈组织、CIN和宫颈癌中的阳性率逐渐升高,差异有统计学意义(P﹤0.05),但与年龄、临床分期、病理分级、淋巴转移无关(均P﹥0.05)。(3)在宫颈癌组织中CDC6与HPV16/18感染呈正相关(P﹤0.05),Survivin与CDC6、HPV16/18感染呈正相关(均P﹤0.05)。[结论]宫颈上皮内瘤样病变及宫颈癌组织中CDC6、Survivin表达的改变可能与HPV16/18感染有关,且互相作用,共同影响CIN的发展及宫颈癌的发生。
[Objective] To investigate the expression of CDC6 and Survivin in cervical cancer and their correlation with HPV16 / 18 infection in order to explore the mechanism of cervical cancer and further search for new molecular markers in diagnosis of cervical cancer, Diagnosis and prognosis. [Methods] Immunohistochemical method was used to detect CDC6 and Survivin in 50 cases of cervical cancer, 20 cases of cervical intraepithelial neoplasia CINⅠ, 20 cases of CINⅡ-Ⅲ and 20 cases of normal cervical tissue. PCR was also used to detect HPV16 / 18 Infection. [Results] (1) The positive rates of CDC6 and Survivin in cervical cancer tissues were higher than those in CIN and normal cervical tissues (all P <0.05). The positive rates of CDC6 and Survivin were correlated with pathological grade, lymph node (All P <0.05). The positive rates of CDC6 and Survivin were not related to age group and clinical stage (all P> 0.05). (2) The positive rate of HPV16 / 18 in normal cervical tissue, CIN and cervical cancer gradually increased, the difference was statistically significant (P <0.05), but not with age, clinical stage, pathological grade, lymph node metastasis > 0.05). (3) There was a positive correlation between CDC6 and HPV16 / 18 infection in cervical cancer (P <0.05). Survivin was positively correlated with CDC6 and HPV16 / 18 infection (all P <0.05). [Conclusion] The changes of CDC6 and Survivin expression in cervical intraepithelial neoplasia and cervical cancer may be related to HPV16 / 18 infection and interact with each other to affect the development of CIN and the occurrence of cervical cancer.