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本研究以体外无血清培养的人早期绒毛细胞滋养层细胞和绒毛组织培养为模型，测定了天花粉蛋白对滋养层细胞ｈＣＧ、孕酮分泌的影响，发现体外无血清培养的细胞滋养层细胞分泌的ｈＣＧ在天花粉蛋白浓度为０．１μｇ／ｍｌ即下降５０％，而后下降缓慢，８μｇ／ｍｌ以上方降为零，而绒毛组织培养在天花粉蛋白浓度为０．１μｇ／ｍｌ时ｈＣＧ下降近９０％，而后下降缓慢，至８μｇ／ｍｌ以上降到零，说明体外培养的细胞滋养层细胞中有两个群体，其中一个对天花粉蛋白敏感，另一个不敏感，尽管在形态上很难区别。孕酮的反应则不同，在细胞滋养层细胞和绒毛组织培养中，随天花粉蛋白浓度升高，孕酮分泌均缓慢下降，未出现两阶段下降过程。
In this study, in vitro cultured human chorionic villi trophoblast cells and chorionic villi tissue culture model for determination of trichosanthin on trophoblast cells hCG, progesterone secretion, found in vitro serum-free culture of trophoblast cells secreted hCG decreased by 50% when the trichosanthin concentration was 0.1μg / ml, then decreased slowly and dropped to zero above 8μg / ml, whereas hCG decreased by 90% when the trichosanthin concentration was 0.1μg / ml, Then slowed down to 8μg / ml or more to zero, indicating that there are two groups of cells in vitro cultured trophoblast cells, one of which is sensitive to trichosanthin, the other is not sensitive, although it is difficult to distinguish morphologically. Progesterone reaction is different, in the trophoblast cells and villi tissue culture, with the increase in the concentration of TCS, progesterone secretion were slowly decreased, there is no two-stage decline.