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目的:从防治溴氰菊酯(deltamethrin,DM)所致妊娠缺失方面研究补肾健脾复方助孕3号对DM生殖毒性的干预作用并初步阐明作用机制。方法:妊娠大鼠分5组,除空白对照组外,其中4组妊娠第1-15d予6.93mg/kgDM经口染毒,分别灌服生理盐水、成人等效、2倍、4倍剂量中药。第15d杀死半数母鼠,余下一半继续妊娠至分娩。观察流产率、活胎数、分娩情况,及雌二醇、孕激素水平。结果:①模型组流产率为30.77%,与其他组差异有显著性。中药高、中、低剂量组流产率分别为8.33、0、16.67%。②各组母鼠均在20-23d内分娩,分娩时未见异常。③妊娠中期(D15),模型组活胎率为(80.67±19.39)%,孕酮为(87.54±20.49)nmol/L,明显低于其他各组。E2水平各组间无明显差异。④分娩后,模型组P及E2水平均明显低于其他各组。结论:DM具有生殖毒性,可致妊娠丢失,其机制与影响母鼠内分泌功能有关,但不影响母鼠分娩。助孕3号方可干预DM毒性,降低流产率,可能与其内分泌调节作用有关。
Objective: To study the intervention effect of Bushen Jianpi Compound Zhuyun 3 on DM reproductive toxicity from prevention and treatment of deltamethrin (DM) induced pregnancy loss and to clarify the mechanism of action. Methods: The pregnant rats were divided into 5 groups. Except for the blank control group, 4 pregnant rats were orally administered with 6.93 mg / kg DM from the 1st to the 15th day of gestation and were given normal saline, . Fifteen days to kill half of maternal mice, the remaining half to continue pregnancy to childbirth. Abortion rates, live births, delivery status, and estradiol and progesterone levels were observed. Results: ① The abortion rate in model group was 30.77%, which was significantly different from other groups. The abortion rates of high, medium and low dose groups of Chinese medicine were 8.33,0,16.67% respectively. ② each group of mothers within 20-23d childbirth, no abnormalities during childbirth. ③ In the second trimester of pregnancy (D15), the rate of live fetus in model group was (80.67 ± 19.39)% and that of progesterone was (87.54 ± 20.49) nmol / L, which was significantly lower than other groups. There was no significant difference between E2 levels in each group. ④ after delivery, the model group P and E2 levels were significantly lower than the other groups. Conclusion: DM has reproductive toxicity and can cause loss of pregnancy. The mechanism is related to the endocrine function of the female rats, but does not affect the delivery of the female rats. Pregnancy 3 may interfere with DM toxicity, reduce abortion rate, may be related to its role in endocrine regulation.