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对红枫湖沉积物中有机碳,孔隙水中的SO42?,以及沉积物中的DNA和类脂化合物的分布进行了研究.红枫湖沉积物有机碳的含量(23.3~76.8mg·g?1)从上到下呈下降趋势,0~8cm含量最高.沉积物孔隙水中SO42?含量为0.89~40.50mg·L?1,表层4cm深度内迅速下降至12mg·L?1,4cm后基本不变.硫酸盐还原指数代表硫酸盐还原细菌对硫酸盐的还原强度,表征SO42?作为一种电子受体在有机质降解过程中被利用的程度.对硫酸盐还原指数SRI的计算表明有机质保留年限为14年,与孔隙水中SO42?含量相对应.沉积物中微生物的总DNA凝胶图像显示,DNA在0~9cm含量相对较高,9cm后相对较低,与有机碳的变化规律和SRI值一致,表明微生物在湖泊沉积物有机质降解过程中发挥了重要作用;缺氧条件下,SO42?是重要的电子受体被微生物利用;沉积物中微生物的总DNA分析为分子生物地球化学研究能为湖泊营养元素循环及湖泊富营养盐化的研究提供重要手段.
The distribution of organic carbon and SO42- in pore water and the DNA and lipid compounds in the sediments of Hongfeng Lake were studied.The content of organic carbon in the sediments of Hongfeng Lake (23.3-76.8 mg · g -1 ) Showed a downward trend from top to bottom, and the content of 0 ~ 8cm was the highest.The content of SO42? In the pore water of sediment was 0.89 ~ 40.50mg · L? 1, the surface layer rapidly decreased to 12mg · L? . Sulfate reduction index represents the reducing strength of sulfate reducing bacteria against sulfate and characterize the extent to which SO42? Is utilized as an electron acceptor in the degradation of organic matter.The calculation of sulfate reduction index (SRI) indicates that the retention time of organic matter is 14 Year, corresponding to the content of SO42- in pore water.The total DNA gel images of the microorganisms in the sediments showed that the content of DNA was relatively high at 0-9 cm and relatively low at 9 cm, which was consistent with the change law of organic carbon and the SRI value, Indicating that microorganisms play an important role in the degradation of organic matter in lacustrine sediments. Under hypoxic conditions, SO42- is an important electron acceptor used by microorganisms. Total DNA analysis of sediment microorganisms can be used as molecular biogeochemistry to study lake nutrition Element cycle and lake rich camp Salinization research provides an important means.