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目的:从衰老动物的氧自由基清除功能出发,探讨益气活血法延缓衰老的作用机理。方法:应用益气活血法治疗气虚血瘀动物(24月龄自然衰老大鼠),用丙二醛法检测血、脑、肝、脾脏、肾上腺过氧化脂质(LPO),放射免疫药盒法测定超氧化物歧化酶(SOD)。结果:血、脑、脾脏、肾上腺的LPO明显降低,血、脑、肝、肾上腺的SOD明显提高。结论:益气活血法能有效地清除神经、内分泌、免疫系统组织器官内的氧自由基,有较强的抗氧化及延缓组织细胞衰老的作用。
OBJECTIVE: To explore the mechanism of the action of invigorating qi and promoting blood circulation to delay aging from the function of oxygen free radical scavenging in aged animals. Methods: Qi-deficiency and blood stasis animals (24-month-old aged rats) were treated with the method of invigorating qi and activating blood circulation. Blood, brain, liver, spleen and adrenal lipid peroxidation (LPO) Determination of superoxide dismutase (SOD). Results: LPO in blood, brain, spleen and adrenal gland were significantly decreased, while SOD in blood, brain, liver and adrenal gland were significantly increased. Conclusion: Yiqihuoxue can effectively remove nerve, endocrine and oxygen free radicals in tissues and organs of the immune system and has strong antioxidation and delayed cell senescence.