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目的:通过HPLC方法测定排石利胆片中芍药苷的含量。方法:选择Hypersil CN(4.6mm×250mm,5μm)为色谱柱,流动相:乙腈-0.1%磷酸溶液(20:80),检测波长:230nm,进样量10μl,1.0m L/min流速,柱温为室温(25℃)条件下进行检测。结果:芍药苷在1.14ug·m L-1~57.00ug·m L-1范围内线性关系良好,线性方程为:Y=238459X-334145(r=0.9992),平均回收率为100.02%,RSD 1.6%。结论:高效液相色谱方法操作简单、结果准确、灵敏度高,可有效评估排石利胆片中芍药苷的含量。
Objective: To determine the content of paeoniflorin in Paishi Decoction by HPLC method. Methods: Hypersil CN (4.6mm × 250mm, 5μm) was used as the column. The mobile phase was acetonitrile-0.1% phosphoric acid solution (20:80), the detection wavelength was 230nm, the injection volume was 10μl and the flow rate was 1.0m L / Temperature is room temperature (25 ℃) conditions for testing. Results: Paeoniflorin showed a good linearity in the range of 1.14ug · m L-1 ~ 57.00ug · m L-1 with a linear equation of Y = 238459X-334145 (r = 0.9992) with an average recovery of 100.02% and RSD 1.6 %. Conclusion: HPLC method is simple, accurate, sensitive and effective for the determination of paeoniflorin in Paishi Li Dan tablets.