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目的探讨小鼠皮肤切创愈合过程中Tenascin表达变化及其与损伤时间的关系。方法制作小鼠背部全层皮肤切创模型,于伤后1、3、6、12h,1、3、6、9、12d分别观察切创周围皮肤、创缘皮肤及创腔肉芽组织的Tenascin免疫组化染色反应,并用图像分析技术检测Tenascin阳性染色的平均光密度,所得数据用SPSS for Windows 10.0软件包进行统计学分析。对照用无切创皮肤。结果对照组仅见真皮乳头、毛囊免疫组化染色弱阳性反应;伤后3h在切创周围的真皮乳头及毛囊Tenascin阳性染色增加;伤后12h明显增强,1~3d达最大值(0.336±0.061;0.282±0.017);伤后6d染色强度开始减弱,9~12d基本恢复正常。创缘皮肤及创腔肉芽组织伤后3~6h开始出现Tenascin阳性着色,12h明显增强,6d达最大值(0.410±0.051;0.460±0.027);伤后9d阳性着色开始减弱,12d基本恢复正常。结论小鼠皮肤切创愈合过程中产生的Tenascin随损伤时间呈现一定的规律性变化。
Objective To investigate the changes of Tenascin expression during incised wound healing in mice and its relationship with injury time. Methods Full-thickness skin incision model was made on the back of mice, and Tenascin immunization was performed on the skin of the incised wounds, the wounds of the wounds and the granulation tissue of wound cavity at 1, 3, 6, 12h, 1, 3, The histochemical staining reaction was performed and the average optical density of Tenascin positive staining was detected by image analysis. The data were statistically analyzed by SPSS for Windows 10.0 software package. Control with no incision skin. Results The dermal papilla and the immunohistochemical staining of hair follicle were weakly positive in the control group. The positive staining of Tenascin in dermal papilla and hair follicle around the incision wound increased 3 h after injury, and significantly increased at 12 h and reached the maximum at 1 to 3 days (0.336 ± 0.061; 0.282 ± 0.017). On the 6th day after injury, the staining intensity began to weaken and returned to normal after 9-12 days. Tenascin positive staining began to occur 3 ~ 6h after wounding and wound granulation tissue injury. The positive staining of Tenascin began at 12h and reached the maximum at 6d (0.410 ± 0.051; 0.460 ± 0.027). The positive staining tended to be weakened at 9d and recovered to normal after 12d. Conclusion Tenascin produced during incised wound healing in mice shows some regular changes with time.