论文部分内容阅读
目的改进动脉内置线阻断法建立的大鼠大脑中动脉（ＭＣＡ）局灶性脑缺血模型，并探讨脑缺血诱发细胞凋亡的时效和量效关系。方法以顶端细，后逐渐增粗的涂抹硅橡胶改进栓子，ＴＴＣ染色鉴定缺血效果；ＴＵＮＥＬ－ＡＰ法和ＨＥ染色观察凋亡发生和形态学改变。结果ＴＴＣ染色证实了大脑缺血灶的的稳定出现；用ＴＵＮＥＬ法发现，缺血３０ｍｉｎ再灌流６ｈ后，凋亡阳性细胞即明显增多，４８ｈ再灌流后阳性细胞数最多；缺血５ｍｉｎ再灌流４８ｈ缺血脑区的凋亡细胞主要位于纹状体，１５ｍｉｎ缺血组皮层也开始散见阳性细胞；随缺血时间延长，凋亡细胞主要出现在缺血区周边。结论脑缺血可选择性诱发神经细胞凋亡。
Objective To improve the model of middle cerebral artery occlusion (MCAO) induced by intra-arterial occlusion in rats and explore the relationship between the time-effect and dose-response of cerebral ischemia-induced apoptosis. Methods The top of the thin, thickening after the application of silicon rubber to improve the emboli, TTC staining to identify the effect of ischemia; TUNEL-AP and HE staining observed apoptosis and morphological changes. Results TTC staining confirmed the stable appearance of cerebral ischemic foci. TUNEL assay showed that the number of apoptotic positive cells increased significantly after reperfusion for 30 min at ischemia for 30 min, and reached the peak at 48 h after reperfusion. Reperfusion was performed 48 h after ischemia Apoptotic cells in the ischemic brain region were mainly located in the striatum, and the positive cells were also seen in the cortex in the ischemic group at 15 min. As the ischemic time prolonged, the apoptotic cells mainly appeared in the periphery of the ischemic area. Conclusion Cerebral ischemia can selectively induce neuronal apoptosis.