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目的 探讨保定地区肾综合征出血热 (HFRS)流行高峰的原因。方法 RT RCR法分型、病毒分离、鉴定及核苷酸序列测定。结果 31例急性期病人血清经RT PCR分型 ,2例为姬鼠型 (HTN型 ) ,2 4例为家鼠型 (SEO型 )。对其中 1例SEO型病毒进行分离、鉴定及其部分M基因的核苷酸序列测定 ,并与SEOUL、R2 2 两株病毒M基因核苷酸及氨基酸同源性比较分别为 :95 .15 %、93 .94%、97.2 7%及97.2 7%。结论 (1)所分离SEO型汉坦病毒为 1996流行高峰年的主要流行株。 (2 )未发现病毒基因片段的重排 ,基因重排变异似不是造成该发病高峰的原因。 (3)不同地区存在的SEO型病毒分子结构差异相对较小 ,较为稳定
Objective To investigate the causes of HFRS epidemic peak in Baoding area. Methods RT RCR typing, virus isolation, identification and nucleotide sequence determination. Results The sera from 31 patients with acute phase were classified by RT PCR. Two were apodemus type (HTN type), and twenty four were mouse type (SEO type). One of the cases of SEO virus was isolated, identified and its partial M gene nucleotide sequence determination, and SEOUL, R2 2 two virus M gene nucleotide and amino acid homology comparison were: 95.15% , 93.94%, 97.2 7% and 97.2 7% respectively. Conclusions (1) The SEO-type Hantaan virus isolated was the main epidemic strain in 1996 peak year. (2) The rearrangement of the viral gene fragment was not found, and the gene rearrangement variation did not appear to be the cause of the peak incidence. (3) The molecular structure of SEO virus in different regions is relatively small and stable