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恙虫病立克次体（Ｒｉｃｋｅｔｔｓｉａｔｓｕｔｓｕｇａｍｕｓｈｉ，Ｒ．ｔ）表面蛋白５６ＫＤａ（ｔｓａ５６ＫＤａ）基因编码区构建的群特异引物，采用嵌合式聚合酶链反应（ＮｅｓｔｅｄＰｏｌｙｍｅｒａｓｅＣｈａｉｎＲｅａｃｔｉｏｎ，ＮＰＣＲ）检测现场捕获的单个小盾纤恙螨幼虫体内Ｒ．ｔ的ＤＮＡ。共检测江苏省恙虫病疫区小盾纤恙螨幼虫６１只，结果２只幼虫提取的ＤＮＡ经扩增后见４８１～５０７ｂｐ的ＤＮＡ扩增带，表明这２只幼虫携带有Ｒ．ｔ，其该种螨Ｒ．ｔ携带率为３．２７％。证明该法可用于单个恙螨幼虫体内Ｒ．ｔ的检测，对恙虫病疫区媒介恙螨的流行病学调查具有实用价值。
A group-specific primer was constructed based on the coding region of the 56 kDa (tsa56KDa) gene of Rickettsiatsutsugamushi (R.t.) surface protein. Nested Polymerase Chain Reaction (NPCR) Larvae in vivo R. t of DNA. A total of 61 larvae of chigger mite were screened for tsutsugamushi disease in Jiangsu Province. As a result, 481 ~ 507bp DNA amplification bands were observed in the DNA extracted from two larvae, indicating that the two larvae carry R. t, the kind of mites R. t carrying rate of 3.27%. It is proved that this method can be used in the body of a single chigger mite larvae. t test, the epidemic of tsutsugamushi disease epidemic chigger mites epidemiological investigation has practical value.