为了从桃叶片中提取到高质量的RNA,以进行反转录、RT-PCR等后续分子生物学试验,选取了改良硼砂-CTAB法、改良CTAB法、改良SDS-苯酚法、试剂盒法、Trizol法,以及在试验中探索的改良Trizol法Ⅰ和改良Trizol法Ⅱ对桃不同发育时期的叶片总RNA进行提取,测定RNA样品的OD值,结合琼脂糖凝胶电泳检测总RNA样品的质量。 In order to extract high quality RNA from peach leaves, we performed the following molecular biology experiments such as reverse transcription, RT-PCR and so on. We chose improved BTA-CTAB method, modified CTAB method, modified SDS- Trizol method and the improved Trizol method I and Trizol method II explored in the experiment were used to extract the total RNA of leaves at different developmental stages. The OD values ​​of RNA samples were determined. The total RNA samples were detected by agarose gel electrophoresis.