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观察８－氯腺苷对人白血病细胞ＨＬ－６０和人Ｔ淋巴母细胞白血病细胞株（Ｍｏｌｔ－４）的分化和凋亡作用以及两种诱导效应的关系。流式细胞术－ＤＮＡ（ＦＣＭ－ＤＮＡ）测定显示８－氯腺苷使ＨＬ－６０和人Ｔ淋巴母细胞白血病细胞株Ｍｏｌｔ－４阻滞在Ｇ０／Ｇ１期。８－氯腺苷诱导人急性粒白血病细胞ＨＬ－６０分化，表现为硝基蓝四氮唑（ｎｉｔｒｏｂｌｕｅｔｅｔｒａｚｏｌｉｕｍ，ＮＢＴ）阳性细胞增多及出现形态学分化表型。通过形态学观察，ＦＣＭ－ＤＮＡ测定及琼脂糖电泳分析结果表明，８－氯腺苷可以诱导Ｍｏｌｔ－４细胞凋亡，且有时间、剂量依赖性，但未发现８－氯腺苷对ＨＬ－６０细胞有诱导凋亡的作用。结论为细胞分化和凋亡是两个并无必然联系的过程
Observed the effect of 8-chloroadenosine on the differentiation and apoptosis of human leukemia cell line HL-60 and human T lymphoblastoid leukemia cell line (Molt-4) and the relationship between the two induction effects. Flow cytometric-DNA (FCM-DNA) assay showed that 8-chloroadenosine blocked HL-60 and the human T-lymphoblastic leukemia cell line Molt-4 in G0/G1 phase. 8-Chloro-adenosine induces the differentiation of human acute granulocyte leukemia cells HL-60, showing an increase in nitroblue tetrazolium (NBT) positive cells and a morphologically differentiated phenotype. Through morphological observation, FCM-DNA assay and agarose gel electrophoresis analysis showed that 8-chloroadenosine could induce apoptosis of Molt-4 cells in a time- and dose-dependent manner, but no 8-chloroadenosine was detected on HL- 60 cells have the effect of inducing apoptosis. Conclusions There are two processes that are not necessarily linked to cell differentiation and apoptosis.