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纳米粒子作为酶固定化的载体,当其具有磁性时,制备的固定化酶易从反应体系中分离和回收,操作简便;并且利用外部磁场可以控制磁性材料固定化酶的运动方式和方向,替代传统的机械搅拌方式,提高固定化酶的催化效率。在众多纳米材料中,氧化铁因其在磁性、催化等多方面的良好特性而备受瞩目。本文对近年来各种氧化铁磁性纳米粒子固定化酶,尤其是固定化脂肪酶和蛋白酶的制备方法及其应用做了较为详细的阐述,对这些氧化铁磁性纳米粒子固定化酶的优缺点和发展前景进行了讨论。
When the nanoparticles are used as the carrier for enzyme immobilization, the prepared immobilized enzyme is easy to be separated and recovered from the reaction system, and the operation is convenient. The external magnetic field can control the movement mode and direction of the immobilized enzyme by the magnetic material, The traditional way of mechanical stirring, improve the catalytic efficiency of immobilized enzyme. Among many nanomaterials, iron oxide has drawn much attention because of its good properties in many aspects such as magnetism and catalysis. In this paper, in recent years, various ferric oxide magnetic nanoparticles immobilized enzymes, especially immobilized lipase and protease preparation method and its application in a more detailed description of these iron oxide magnetic nanoparticles immobilized enzyme advantages and disadvantages and Development prospects are discussed.