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以丙烯酰胺为单体,采用原位聚合法制备了Fe3O4/聚丙烯酰胺纳米磁粒(Fe3O4/PAM);利用胺基与金的相互作用,借助自组装法在Fe3O4/PAM表面组装金胶体制备了草莓型纳米金磁颗粒(Fe3O4/PAM/Au);用TEM、VSM、UV-vis对其进行了表征,并考察了表面修饰核酸探针的金磁颗粒对核酸靶分子的分离能力。结果表明,Fe3O4/PAM/Au粒子的粒径为36～56nm,具有超顺磁性,饱和磁化强度为31.2emu/g,分散在磷酸盐缓冲液中的Fe3O4/PAM/Au完全磁分离的时间为6min。修饰核酸探针的Fe3O4/PAM/Au粒子可以借助核酸杂交作用分离核酸靶分子,分离能力为118pmol/mg。
Fe3O4 / polyacrylamide nano-magnetic particles (Fe3O4 / PAM) were prepared by in-situ polymerization using acrylamide as monomer. The gold colloids were prepared by the self-assembly method using the interaction of amine groups and gold Strawberry-type nano-gold particles (Fe3O4 / PAM / Au) were synthesized and characterized by TEM, UV-vis and VSM. The separation ability of the gold particles with surface-modified nucleic acid probes on the target was also investigated. The results show that the particle size of Fe3O4 / PAM / Au particles is 36-56nm with superparamagnetism and the saturation magnetization is 31.2emu / g. The time of complete magnetic separation of Fe3O4 / PAM / Au dispersed in phosphate buffer solution is 6min. The nucleic acid probe modified Fe3O4 / PAM / Au particles can be separated by nucleic acid hybridization of nucleic acid target molecules, the separation capacity of 118 pmol / mg.