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①目的探讨单纯性肾病综合征（ＳＮＳ）病儿血清可溶性白细胞介素２受体（ｓＩＬ－２Ｒ）的变化特点及其临床意义。②方法采用双抗体夹心ＥＬＩＳＡ法和单克隆抗体间接免疫荧光法，检测２４例ＳＮＳ病儿活动期、近期和远期缓解期及２２例健康儿童血清ｓＩＬ－２Ｒ水平和Ｔ细胞亚群。③结果ＳＮＳ病儿活动期血清ｓＩＬ－２Ｒ水平明显高于近期和远期缓解期及正常对照组（ｔ＝５．６８１～６．３６１，Ｐ＜０．０１），而其他各组间差异无显著性（ｔ＝０．２３３～０．６０１，Ｐ＞０．０５）；活动期血清ｓＩＬ－２Ｒ增高与ＣＤ４细胞百分率和ＣＤ４／ＣＤ８细胞比值呈负相关（ｒ＝－０．４９８，－０．６２５，Ｐ＜０．０５，０．０１），而与ＣＤ８细胞百分率呈正相关（ｒ＝０．５１７，Ｐ＜０．０１）；激素敏感组活动期血清ｓＩＬ－２Ｒ水平明显低于低敏感组（ｔ＝２．２７６，Ｐ＜０．０５），活动期血清ｓＩＬ－２Ｒ水平与尿蛋白阴转时间呈低度正相关（ｒ＝０．４３３，Ｐ＜０．０５）。④结论ＳＮＳ病儿活动期血清ｓＩＬ－２Ｒ水平明显增高，此与Ｔ细胞功能紊乱和ＳＮＳ发病机制密切相关；活动期血清ｓＩＬ－２Ｒ水平可作为判断激素近期疗效的指标。
Objective To investigate the changes of serum soluble interleukin-2 receptor (sIL-2R) in children with simple nephrotic syndrome (SNS) and its clinical significance. Methods Serum levels of sIL-2R and T lymphocyte subsets in 24 SNS patients during active, immediate and long-term remission, and 22 healthy children were detected by double antibody sandwich ELISA and indirect immunofluorescence. Results The level of serum sIL-2R in patients with SNS was significantly higher than those in the immediate and long-term remissions and the normal control group (t = 5.681-6.361, P <0.01), while the other groups had no difference (T = 0.233-0.601, P> 0.05). The increase of serum sIL-2R in active stage was negatively correlated with the percentage of CD4 cells and the ratio of CD4 / CD8 cells (r = -0.498, -0 .625, P <0.05,0.01), but positively correlated with the percentage of CD8 cells (r = 0.517, P <0.01). The level of serum sIL-2R in active hormone sensitive group was significantly lower than that in low sensitive Group (t = 2.276, P <0.05). There was a low positive correlation between serum sIL-2R level and urinary protein negative conversion time (r = 0.433, P <0.05). Conclusion The serum level of sIL-2R in active stage of SNS patients is significantly increased, which is closely related to the T cell dysfunction and the pathogenesis of SNS. Active serum sIL-2R level may be used as an indicator of the short-term effect of hormone therapy.