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目的:采用HPLC法测定冠脉康浓缩丸中的芍药苷含量。方法:采用高效液相色谱法,色谱柱为岛津C18柱(4.6mm×150mm,5μm),流动相为乙腈-0.1%磷酸溶液(15∶85),流速为1.0mL/min,柱温为40℃,检测波长为230nm。结果:芍药苷在0.005 5~0.044 0mg·mL-1浓度范围内与其峰面积积分呈良好的线性关系,r=0.999 2,平均加样回收率为99.65%,RSD=1.06%。结论:该方法简便可行、重复性好,可用于冠脉康浓缩丸中芍药苷的含量测定,可有效控制该制剂质量。
Objective: To determine the content of paeoniflorin in Guanmaikang concentrated pills by HPLC. Methods: The HPLC column was Shimadzu C18 column (4.6 mm × 150 mm, 5 μm). The mobile phase was acetonitrile-0.1% phosphoric acid solution (15:85), the flow rate was 1.0 mL / min and the column temperature was 40 ℃, the detection wavelength is 230nm. Results: Paeoniflorin had a good linear relationship with the peak area in the range of 0.005 5 ~ 0.044 0 mg · mL-1, r = 0.999 2, the average recovery was 99.65% and the RSD was 1.06%. Conclusion: The method is simple, feasible and reproducible. It can be used to determine the content of paeoniflorin in Zhiganmai Nourishing Pills, which can effectively control the quality of the preparation.