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研究两个反义核酸（18mer，21mer）对人白血病HL—60细胞中c－myc基因表达的抑制作用。用HL－60活细胞计数，RNA含量的RT－PCR测定和c－myc基因蛋白的免疫组化染色检查。结果：两个反义寡核苷酸都能抑制HL－60细胞的增殖，增殖抑制率为11％～75％。反义核酸还抑制c－mycmRNA和Myc蛋白的表达，而对c－mcy基因正常表达的红白血病细胞K562的增殖和PHA刺激的人淋巴细胞的增殖没有影响。结论：反义寡核苷酸能抑制c－myc基因的过度表达。
The inhibitory effect of two antisense nucleic acids (18mer, 21mer) on c-myc gene expression in human leukemia HL-60 cells was investigated. HL-60 viable cell counts, RT-PCR assays of RNA content, and immunohistochemical staining of c-myc gene protein were examined. RESULTS: Both antisense oligonucleotides inhibited the proliferation of HL-60 cells and the proliferation inhibition rate was 11% to 75%. Antisense nucleic acids also inhibit the expression of c-myc mRNA and Myc protein, but have no effect on the proliferation of erythroleukemia cell K562 and the proliferation of PHA-stimulated human lymphocytes that are normally expressed in the c-mcy gene. Conclusion: Antisense oligonucleotides can inhibit the overexpression of c-myc gene.