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目的:观察低强度复合振动对去卵巢老龄大鼠骨质疏松的治疗作用。方法:13月龄SD雌性经产大鼠16只,随机均分为对照组和实验组。卵巢摘除法骨质疏松造模,3个月后检查骨密度(BMD)下降后实验组接受低强度复合振动(振动频率45 Hz～55 Hz,振动强度0.12 g～0.21 g,g=9.8 m/s2,20分钟/次/天,5天/周),对照组不进行振动治疗。13周复合振动实验结束后分别活体测量两组大鼠腰椎、股骨骨密度(Lorland XR-46),胫骨标本骨微结构扫描分析(SCANCO u-CT),测试腰椎、股骨标本力学性能(858 mini bionix)及酶法检测血清碱性磷酸酶、非前列腺酸性磷酸酶,电化学发光免疫法检测血清骨钙素和I型胶原C端交联肽。结果:复合振动实验结束后实验组腰椎和股骨BMD较实验前显著增加(分别增加0.0110±0.0048 g/cm2,P=0.000和0.0056±0.0065 g/cm2,P=0.004),而对照组显著下降(分别下降0.0071±0.0042 g/cm2和0.0073±0.0063 g/cm2)。相较对照组,骨微结构参数Tb.N显著增加(P=0.002)、Tb.Sp显著降低(P=0.005)以及BV/TV显著增加(P=0.048),腰椎、股骨强度极限也显著增加(P=0.038,P=0.014),但血清骨钙素、碱性磷酸酶、I型胶原C端交联肽、非前列腺酸性磷酸酶两组无统计学差异。结论:低强度复合振动可以减轻去卵巢老龄大鼠骨质疏松程度,提高骨质疏松大鼠骨质量。
Objective: To observe the therapeutic effect of low-intensity compound vibration on osteoporosis in ovariectomized aged rats. Methods: Thirteen SD female rats of 13 months old were randomly divided into control group and experimental group. Ovariectomized osteoporosis model 3 months after the examination of bone mineral density (BMD) decline in the experimental group to accept low-intensity composite vibration (vibration frequency 45 Hz ~ 55 Hz, vibration intensity 0.12 g ~ 0.21 g, g = 9.8 m / s2, 20 minutes / time / day, 5 days / week), the control group did not carry out vibration treatment. After 13 weeks of combined vibration test, lumbar and femur BMD (Lorland XR-46) and SCANCO u-CT scan of the lumbar spine and femur specimens were measured in vivo. Serum alkaline phosphatase and non-prostatic acid phosphatase were detected by enzyme-linked immunosorbent assay (ELISA) and C-terminal crosslinked peptide of type I collagen by radioimmunoassay. Results: BMD of lumbar spine and femur increased significantly (0.0110 ± 0.0048 g / cm2, P = 0.000 and 0.0056 ± 0.0065 g / cm2, P = 0.004, respectively) in the experimental group at the end of the compound vibration test Decreased by 0.0071 ± 0.0042 g / cm 2 and 0.0073 ± 0.0063 g / cm 2, respectively). Compared with the control group, Tb.N increased significantly (P = 0.002), Tb.Sp decreased significantly (P = 0.005) and BV / TV increased significantly (P = 0.048), and the strength of lumbar spine and femur increased significantly (P = 0.038, P = 0.014). However, there was no significant difference between the two groups in serum osteocalcin, alkaline phosphatase, type C collagen C-terminal cross-linking peptide and non-prostatic acid phosphatase. Conclusion: Low-intensity composite vibration can reduce the degree of osteoporosis in ovariectomized aged rats and improve the bone quality of rats with osteoporosis.