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目的:采用Folin-Ciocalteu比色法测定枸杞子中总酚酸的含量。方法:以绿原酸作为对照品,以Folin-Ciocalteu试剂为显色剂,检测波长为761nm。结果:浓度为0.00318～0.0212mg/mL时与吸光度呈良好线性关系,测定方法的平均回收率为100.8%,相对标准偏差(RSD)为0.71%。结论:此法操作简便,准确可靠,为枸杞子药材的质量控制、综合开发利用提供了一定的方法和依据。
Objective: Folin-Ciocalteu colorimetry was used to determine total phenolic acids in Fructus Lycii. Methods: Chlorogenic acid was used as a reference substance and Folin-Ciocalteu reagent was used as a chromogenic reagent. The detection wavelength was 761 nm. Results: There was a good linear relationship between the absorbance and the concentration of 0.00318 ~ 0.0212mg / mL. The average recovery was 100.8% and the relative standard deviation (RSD) was 0.71%. Conclusion: This method is simple, accurate and reliable, which provides a certain method and basis for quality control and comprehensive development and utilization of wolfberry fruit.