目的：检测健康人群血清莱姆病抗体水平。方法：间接免疫荧光抗体试验（ＩＦＡ）、酶联免疫吸附试验（ＥＬＩＳＡ）。结果：共检测２１８４人，阳性２２５人，感染率１０３０％。儿童（１３８５％）、学生（１１９３％）感染率最高，农民（１０５７％）次之，林业工人及管理人员（６４４％）相对较低；林区（１０３７％）与非林区（１０２７％）人群感染率差异无显著性（χ２＝０２１，Ｐ＞０７５）。１８０例莱姆病血清阳性者中，蜱叮咬率为４０％，未发现典型的慢性游走性红斑（ＥＣＭ）皮疹患者，红斑、风团、紫斑占３２７８％，肌痛占２３３３％，关节炎占４１１１％。结论：粤东地区人群莱姆病感染率较高 Objective: To detect serum levels of Lyme disease in healthy population. Methods: Indirect immunofluorescence antibody test (IFA), enzyme-linked immunosorbent assay (ELISA). Results: A total of 2184 people were tested, 225 were positive, the infection rate was 1030%. The infection rate of children (1385%) and students (1193%) was the highest, followed by farmer (1057%), forestry workers and management staff (644%), while that of forest area (10.37% %) And non-forest area (1027%) were no significant difference (χ2 = 021, P> 075). Among 180 cases of Lyme seropositive, tick bite rate was 40%. No typical erythematous erythematosus (ECM) rash was found in patients with erythema, typhoid, and purpura (32.78%) and myalgia (23.33%) %, Arthritis accounted for 4111%. Conclusion: The prevalence of Lyme disease in eastern Guangdong is high